Publications

Kishore, S.; Privalsky, T. M.; Del Rio Flores, A.; Lynch, S. R.; Khosla, C. Self‑Resistance Guided Discovery of a Hybrid Polyketide‑Peptide Antibiotic from Vibrio ruber. J. Am. Chem. Soc. ASAP (published June 2025), DOI: 10.1021/jacs.5c07625.

Del Rio Flores, A., & Khosla, C. (2024b, October 28). Biochemical characterization of the Flavin-Dependent monooxygenase required for the biosynthesis of the Nocardiosis-Associated glycolipid. AIChE - 2024 AIChE Annual Meeting. https://aiche.confex.com/aiche/2024/meetingapp.cgi/Paper/682172

Del Rio Flores, A., & Khosla, C. (2024b, October 27). Discovery and characterization of the fully decorated Nocardiosis-Associated polyketide natural product. AIChE - 2024 AIChE Annual Meeting. https://aiche.confex.com/aiche/2024/meetingapp.cgi/Paper/681983

Del Rio Flores, A., & Khosla, C. (2024). Characterization of the Flavin-Dependent monooxygenase involved in the biosynthesis of the Nocardiosis-Associated polyketide. Biochemistry, 63(21), 2868–2877. https://doi.org/10.1021/acs.biochem.4c00480

Del Rio Flores, A., Zhai, R., Kastner, D. W., Seshadri, K., Yang, S., De Matias, K., Shen, Y., Cai, W., Narayanamoorthy, M., B, N., DO, Xue, Z., Marzooqi, D. A., Kulik, H. J., & Zhang, W. (2024). Enzymatic synthesis of azide by a promiscuous N-nitrosylase. Nature Chemistry. https://doi.org/10.1038/s41557-024-01646-2

The Atomwise AIMS Program. AI is a viable alternative to high throughput screening: a 318-target study. Sci Rep 14, 7526 (2024). https://doi.org/10.1038/s41598-024-54655-z

Kishore, S., Del Rio Flores, A., Lynch, S. R., Yuet, K. P., & Khosla, C. (2024). Discovery and characterization of the fully decorated Nocardiosis-Associated polyketide natural product. Journal of the American Chemical Society, 146(6), 4212–4220. https://doi.org/10.1021/jacs.3c13670

Del Rio Flores, A., Zhai, R., & Zhang, W. (2024). Isonitrile biosynthesis by non-heme iron(II)-dependent oxidases/decarboxylases. Methods in Enzymology on CD-ROM/Methods in Enzymology, 143–172. https://doi.org/10.1016/bs.mie.2024.06.002

Del Rio Flores, A., & Zhang, W. (2023, November 9). Discovery of a Pathway for Azide Biosynthesis By a Promiscuous N-Nitrosylase. AIChE - 2023 AIChE Annual Meeting. https://aiche.confex.com/aiche/2023/meetingapp.cgi/Paper/662003

Del Rio Flores, A., Narayanamoorthy, M., Cai, W., Zhai, R., Yang, S., Shen, Y., Seshadri, K., De Matias, K., Xue, Z., & Zhang, W. (2023). Biosynthesis of Isonitrile Lipopeptide Metallophores from Pathogenic Mycobacteria. Biochemistry, 62(3), 824–834. https://doi.org/10.1021/acs.biochem.2c00611

Del Rio Flores, A., & Zhang, W. (2023a, January 9). BIOSYNTHESIS OF UNUSUAL SYNTHONS IN NATURAL PRODUCTS. SIMB - 4th International Conference on Natural Product Discovery and Development in the Genomic Era. https://sim.confex.com/sim/np2023/meetingapp.cgi/Paper/46086

Antonio, D. R. F. (2023). Biosynthesis of unusual synthons in natural products. https://escholarship.org/uc/item/21x7055c

Flores, A. D. R., Kastner, D., & Zhang, W. (2023, January). Structure and Dynamics Perspectives on Enzyme Function Probing the mechanism of isonitrile formation by a non-heme iron (II)-dependent oxidase/decarboxylase. In PROTEIN SCIENCE (Vol. 32). 111 RIVER ST, HOBOKEN 07030-5774, NJ USA: WILEY.

Del Rio Flores, A., Barber, C. C., Narayanamoorthy, M., Gu, D., Shen, Y., & Zhang, W. (2022). Biosynthesis of Isonitrile- and Alkyne-Containing natural products. Annual Review of Chemical and Biomolecular Engineering, 13(1), 1–24. https://doi.org/10.1146/annurev-chembioeng-092120-025140

Del Rio Flores, A., Kastner, D. W., Du, Y., Narayanamoorthy, M., Shen, Y., Cai, W., Vennelakanti, V., Zill, N. A., Dell, L. B., Zhai, R., Kulik, H. J., & Zhang, W. (2022). Probing the mechanism of isonitrile formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase. Journal of the American Chemical Society, 144(13), 5893–5901. https://doi.org/10.1021/jacs.1c12891

Zhang, H., Goh, N.S., Wang, J.W. et al. Nanoparticle cellular internalization is not required for RNA delivery to mature plant leaves. Nat. Nanotechnol. 17, 197–205 (2022). https://doi.org/10.1038/s41565-021-01018-8

Del Rio Flores A., Twigg, F.F., Du, Y. et al. Biosynthesis of triacsin featuring an N-hydroxytriazene pharmacophore. Nat Chem Biol 17, 1305–1313 (2021). https://doi.org/10.1038/s41589-021-00895-3

Del Rio Flores, A., Twigg, F. F., Du, Y., Cai, W., Aguirre, D. Q., Sato, M., Dror, M. J., Narayanamoorthy, M., Geng, J., Zill, N. A., & Zhang, W. (2021). Total biosynthesis of triacsin featuring an N-hydroxytriazene pharmacophore. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2021.05.12.443849

Del Rio Flores, A., Twigg, F. F., Du, Y., Cai, W., Aguirre, D. Q., Sato, M., Dror, M. J., Narayanamoorthy, M., Geng, J., Zill, N. A., & Zhang, W. (2021b). Total biosynthesis of triacsin featuring an N-hydroxytriazene pharmacophore. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2021.05.12.443849

Jonnalagadda, R., Del Rio Flores, A., Cai, W., Mehmood, R., Narayanamoorthy, M., Ren, C., Zaragoza, J. P., Kulik, H. J., Zhang, W., & Drennan, C. L. (2021). Biochemical and crystallographic investigations into isonitrile formation by a nonheme iron-dependent oxidase/decarboxylase. Journal of Biological Chemistry, 296, 100231. https://doi.org/10.1074/jbc.ra120.015932

Jonnalagadda, R., Del Rio Flores, A., & Cai, W. (2021). Biochemical and crystallographic investigations into isonitrile formation by a nonheme iron‑dependent oxidase/decarboxylase. Journal of Biological Chemistry, 296, Article 100231.

Li, J. S., Du, Y., Gu, D., Cai, W., Green, A., Ng, S., Leung, A., Del Rio Flores, A., & Zhang, W. (2020). Discovery and Biosynthesis of Clostyrylpyrones from the Obligate Anaerobe Clostridium roseum. Organic Letters, 22(21), 8204–8209. https://doi.org/10.1021/acs.orglett.0c02656

Huang, Y.-B., Luo, Y.-J., Rio Flores, A. D., Li, L.-C., & Wang, F. (2020). N-aryl pyrrole synthesis from biomass-derived furans and arylamine over lewis acidic hf-doped mesoporous SBA-15 catalyst. ACS Sustainable Chemistry & Engineering, 8(32), 12161–12167. https://doi.org/10.1021/acssuschemeng.0c03578

Skyrud, W., Del Rio Flores, A., & Zhang, W. (2020a, January 13). Biosynthesis of cyclohexylcarbonyl-CoA highlights a promiscuous shikimate-CoA synthetase and a FAD-dependent dehydratase. SIMB - 3rd International Conference on Natural Products Discovery and Development in the Genomic Era. https://sim.confex.com/sim/np2020/meetingapp.cgi/Paper/40543

Skyrud, W., Del Rio Flores, A., & Zhang, W. (2020). Biosynthesis of Cyclohexanecarboxyl-COA highlights a promiscuous Shikimoyl-COA synthetase and a FAD-Dependent dehydratase. ACS Catalysis, 10(5), 3360–3364. https://doi.org/10.1021/acscatal.0c00406

Del Rio Flores A., Twigg, F.F., Cai W., et al. Facile Discovery and Quantification of Isonitrile Natural Products via Tetrazine-Based Click Reactions. Anal. Chem. 2020, 92, 1, 599–602. https://doi.org/10.1021/acs.analchem.9b05147

Ren, Y., Bai, Y., Zhang, Z., Cai, W., & Del Rio Flores, A. (2019). The Preparation and Structure Analysis Methods of Natural Polysaccharides of Plants and Fungi: A Review of Recent Development. Molecules, 24(17), 3122. https://doi.org/10.3390/molecules24173122

Huang, Y.-B., Cai, W., Del Rio Flores, A. D. R., Twigg, F. F., & Zhang, W. (2020). Facile discovery and quantification of isonitrile natural products via tetrazine-based click reactions. Analytical Chemistry, 92(1), 599–602.